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h1 embryonic stem cell line  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc h1 embryonic stem cell line
    H1 Embryonic Stem Cell Line, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryonic+stem+cell+line+h1/WA01/bio_rxiv__64898__2026__03__09__710481-226-1-5
    Average 99 stars, based on 962 article reviews
    h1 embryonic stem cell line - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
    Article Snippet: .. Cells of the human embryonic stem cell line H1 (WA01 cells, WiCell Research Institute, Madison, Wisconsin) at passage 42 were seeded as single cells at 1×105 cells/cm2 on MATRIGELTM at a 1:30 dilution (Corning Incorporated, Corning New York, Catalog No. 354230) coated dishes in a media of Dulbecco's Modified Eagle's Medium; Nutrient mixture F-12 (“DMEM-F12”) (Life Technologies Corporation, Carlsbad, California, Catalog No. 11330-032), GLUTAMAXTM (Life Technologies, Catalog No. 35050-079) in a 1:100 dilution (“1× concentration”), 0.25 mM ascorbic acid (Sigma Aldrich Co. LLC, St. Louis Missouri, Catalog No. A4544), 100 ng/ml of fibroblast growth factor 2 (“FGF2”) (R & D Systems, Minneapolis, Minnesota, Catalog No. 233-FB-025), 1 ng/ml of transforming growth factor beta (“TGF-β”) (R & D Systems Inc., Catalog No. 240-B-002), insulin-transferrin-selenium-ethanolamine (“ITS-X”) (Life Technologies, Catalog No. 51500056) at a 1:100 dilution, 2% fatty-acid free bovine serum albumin (“FAF-BSA”) (Proliant, Inc., Boone, Idaho, Catalog No. 68700), and 20 ng/ml of insulin-like growth factor-1 (“IGF-1”) (R & D Systems, Catalog No. 291-G1-200), supplemented with 10 μM of Rock inhibitor Y-27632 (Catalog No. Y0503, Sigma-Aldrich). ..

    Concentration Assay:

    Article Title: Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
    Article Snippet: .. Cells of the human embryonic stem cell line H1 (WA01 cells, WiCell Research Institute, Madison, Wisconsin) at passage 42 were seeded as single cells at 1×105 cells/cm2 on MATRIGELTM at a 1:30 dilution (Corning Incorporated, Corning New York, Catalog No. 354230) coated dishes in a media of Dulbecco's Modified Eagle's Medium; Nutrient mixture F-12 (“DMEM-F12”) (Life Technologies Corporation, Carlsbad, California, Catalog No. 11330-032), GLUTAMAXTM (Life Technologies, Catalog No. 35050-079) in a 1:100 dilution (“1× concentration”), 0.25 mM ascorbic acid (Sigma Aldrich Co. LLC, St. Louis Missouri, Catalog No. A4544), 100 ng/ml of fibroblast growth factor 2 (“FGF2”) (R & D Systems, Minneapolis, Minnesota, Catalog No. 233-FB-025), 1 ng/ml of transforming growth factor beta (“TGF-β”) (R & D Systems Inc., Catalog No. 240-B-002), insulin-transferrin-selenium-ethanolamine (“ITS-X”) (Life Technologies, Catalog No. 51500056) at a 1:100 dilution, 2% fatty-acid free bovine serum albumin (“FAF-BSA”) (Proliant, Inc., Boone, Idaho, Catalog No. 68700), and 20 ng/ml of insulin-like growth factor-1 (“IGF-1”) (R & D Systems, Catalog No. 291-G1-200), supplemented with 10 μM of Rock inhibitor Y-27632 (Catalog No. Y0503, Sigma-Aldrich). ..

    Cell Culture:

    Article Title: A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood
    Article Snippet: Cells were washed again with deionized water and then with tap water, and then Eosin (Sigma-Aldrich, St. Louis, MO, USA) was added and incubated for 30 s. Lastly, cells were incubated in ethanol, and the culture slides were studied using a light microscope (Leica DM2000, Leica Microsystems GmbH, Wetzlar, Germany). .. The human embryonic stem cell line H1 (Wicell International Stem Cell Bank, Wicell Research Institute, Madison, WI, USA) was cultured as previously described [ ] and served as a positive control in antibody verification of embryonic stem cell markers. .. H1 stem cells were cultured in 6-well plates coated with Matrigel (BD Biosciences, San Jose, CA, USA), fed daily with mTeSR1 (Stem Cell Technologies, Vancouver, BC, Canada) and observed under a microscope for any spontaneous differentiation.

    Positive Control:

    Article Title: A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood
    Article Snippet: Cells were washed again with deionized water and then with tap water, and then Eosin (Sigma-Aldrich, St. Louis, MO, USA) was added and incubated for 30 s. Lastly, cells were incubated in ethanol, and the culture slides were studied using a light microscope (Leica DM2000, Leica Microsystems GmbH, Wetzlar, Germany). .. The human embryonic stem cell line H1 (Wicell International Stem Cell Bank, Wicell Research Institute, Madison, WI, USA) was cultured as previously described [ ] and served as a positive control in antibody verification of embryonic stem cell markers. .. H1 stem cells were cultured in 6-well plates coated with Matrigel (BD Biosciences, San Jose, CA, USA), fed daily with mTeSR1 (Stem Cell Technologies, Vancouver, BC, Canada) and observed under a microscope for any spontaneous differentiation.

    other:

    Article Title: Low-dose of polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids.
    Article Snippet: The human embryonic stem cell line H1 (H1 ES) (WA01, XY karyotype, WiCell Research institute, USA) ES line was cultivated in mTeSR1 medium (Stemcell Technologies, Canada) on Matrigel (BD science,USA) -coated culture dishes (Corning, USA).

    Article Title: Polystyrene microplastics induce hepatotoxicity and disrupt lipid metabolism in the liver organoids.
    Article Snippet: • The liver organoids (LOs) were a novel model to study microplastics toxicity.. • The cultivation of hPSC-derived LOs and exposure of MP were non-static.. • PS-MPs induced hepatotoxicity and disrupted lipid metabolism in the LOs.



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    a –c For each species: circular projection of CellRank-calculated fate probabilities for each cell toward the terminal states (outer labels); and UMAPs detailing endocrine progenitors branching, with the integrated pancreas atlas showing NEUROG3 expression (red) and endocrine progenitor cluster boundaries (black), and an insert showing endocrine progenitor subclusters with overlaid CellRank-inferred trajectories (arrows). d Line plots showing the cumulative number of CellRank-derived beta-cell (top) and alpha-cell (bottom) lineage drivers in mouse and pig that overlap with human orthologs, plotted across correlation score thresholds (Supplementary Data ). Solid lines show significant driver numbers (Benjamini-Hochberg FDR-corrected p-value < 0.05). Shaded regions indicate the number of genes obtained when using the lower and upper bounds of the 95% confidence interval for the corresponding correlation score. e, f Heatmaps displaying modeled gene expression patterns for human beta-cell ( e ) and alpha-cell ( f ) lineage driver gene clusters (identified by hierarchical clustering) across pig, human, and mouse along pseudotemporal trajectories (left to right: 0 → 1). Annotations indicate species-conserved pathways and representative genes for each cluster. (corr., correlation; n, number of conserved genes that are expressed in > 20% of endocrine progenitor subclusters) g –i Comparison of NEUROG3 TF targets identified in human/pig pancreas and <t>hESC</t> model (conserved targets in blue). g, h Circular GRN graphs showing first- and second-order NEUROG3 targets from human scGLUE-jointed scRNA/ATAC-seq data ( g ) and pig multiomic data ( h ). Nodes represent TFs. Edge color indicates regulatory interaction types (orange, activating; gray, inhibiting); i NEUROG3 TF targets in hESC model with inducible NEUROG3 expression. ChIP-seq-identified direct targets are shaded. Differentially expressed TFs comparing cells with/without NEUROG3 expression are indicated by arrows (red, upregulated; blue, downregulated). a – f scRNA-seq of pancreatic cells from wild-type and INS -eGFP pigs. h Multiome analysis of pancreatic cells from PTF1A -codon-improved-Cre/ROSA-mTmG pigs. Detailed sample information is provided in Supplementary Data .
    Parent Human Embryonic Stem Cell Hesc Line Wa01 H1, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a –c For each species: circular projection of CellRank-calculated fate probabilities for each cell toward the terminal states (outer labels); and UMAPs detailing endocrine progenitors branching, with the integrated pancreas atlas showing NEUROG3 expression (red) and endocrine progenitor cluster boundaries (black), and an insert showing endocrine progenitor subclusters with overlaid CellRank-inferred trajectories (arrows). d Line plots showing the cumulative number of CellRank-derived beta-cell (top) and alpha-cell (bottom) lineage drivers in mouse and pig that overlap with human orthologs, plotted across correlation score thresholds (Supplementary Data ). Solid lines show significant driver numbers (Benjamini-Hochberg FDR-corrected p-value < 0.05). Shaded regions indicate the number of genes obtained when using the lower and upper bounds of the 95% confidence interval for the corresponding correlation score. e, f Heatmaps displaying modeled gene expression patterns for human beta-cell ( e ) and alpha-cell ( f ) lineage driver gene clusters (identified by hierarchical clustering) across pig, human, and mouse along pseudotemporal trajectories (left to right: 0 → 1). Annotations indicate species-conserved pathways and representative genes for each cluster. (corr., correlation; n, number of conserved genes that are expressed in > 20% of endocrine progenitor subclusters) g –i Comparison of NEUROG3 TF targets identified in human/pig pancreas and hESC model (conserved targets in blue). g, h Circular GRN graphs showing first- and second-order NEUROG3 targets from human scGLUE-jointed scRNA/ATAC-seq data ( g ) and pig multiomic data ( h ). Nodes represent TFs. Edge color indicates regulatory interaction types (orange, activating; gray, inhibiting); i NEUROG3 TF targets in hESC model with inducible NEUROG3 expression. ChIP-seq-identified direct targets are shaded. Differentially expressed TFs comparing cells with/without NEUROG3 expression are indicated by arrows (red, upregulated; blue, downregulated). a – f scRNA-seq of pancreatic cells from wild-type and INS -eGFP pigs. h Multiome analysis of pancreatic cells from PTF1A -codon-improved-Cre/ROSA-mTmG pigs. Detailed sample information is provided in Supplementary Data .

    Journal: Nature Communications

    Article Title: A multimodal cross-species comparison of pancreas development

    doi: 10.1038/s41467-025-64774-4

    Figure Lengend Snippet: a –c For each species: circular projection of CellRank-calculated fate probabilities for each cell toward the terminal states (outer labels); and UMAPs detailing endocrine progenitors branching, with the integrated pancreas atlas showing NEUROG3 expression (red) and endocrine progenitor cluster boundaries (black), and an insert showing endocrine progenitor subclusters with overlaid CellRank-inferred trajectories (arrows). d Line plots showing the cumulative number of CellRank-derived beta-cell (top) and alpha-cell (bottom) lineage drivers in mouse and pig that overlap with human orthologs, plotted across correlation score thresholds (Supplementary Data ). Solid lines show significant driver numbers (Benjamini-Hochberg FDR-corrected p-value < 0.05). Shaded regions indicate the number of genes obtained when using the lower and upper bounds of the 95% confidence interval for the corresponding correlation score. e, f Heatmaps displaying modeled gene expression patterns for human beta-cell ( e ) and alpha-cell ( f ) lineage driver gene clusters (identified by hierarchical clustering) across pig, human, and mouse along pseudotemporal trajectories (left to right: 0 → 1). Annotations indicate species-conserved pathways and representative genes for each cluster. (corr., correlation; n, number of conserved genes that are expressed in > 20% of endocrine progenitor subclusters) g –i Comparison of NEUROG3 TF targets identified in human/pig pancreas and hESC model (conserved targets in blue). g, h Circular GRN graphs showing first- and second-order NEUROG3 targets from human scGLUE-jointed scRNA/ATAC-seq data ( g ) and pig multiomic data ( h ). Nodes represent TFs. Edge color indicates regulatory interaction types (orange, activating; gray, inhibiting); i NEUROG3 TF targets in hESC model with inducible NEUROG3 expression. ChIP-seq-identified direct targets are shaded. Differentially expressed TFs comparing cells with/without NEUROG3 expression are indicated by arrows (red, upregulated; blue, downregulated). a – f scRNA-seq of pancreatic cells from wild-type and INS -eGFP pigs. h Multiome analysis of pancreatic cells from PTF1A -codon-improved-Cre/ROSA-mTmG pigs. Detailed sample information is provided in Supplementary Data .

    Article Snippet: The parent human embryonic stem cell (hESC) line WA01 (H1) was obtained from WiCell.

    Techniques: Expressing, Derivative Assay, Gene Expression, Comparison, ChIP-sequencing